3.1 Inspection name
The radical measures the > pituitary hormone test
3.3 Laboratory materials
3.4 Measurement principle of FSH
The measurement of follicular stimulating hormone radioimmunoassay was carried out by unbalanced method. Standard/follicle-stimulating hormone and specific antibody in the sample under test after incubate, follicle-stimulating hormone (FSH) levels were, join the I - 125 when the second incubate the I - 125 follicle-stimulating hormone and follicle-stimulating hormone (FSH) levels were competition in sample limited specific antibody combining site, then add the separating agent separation antigen-antibody complexes and free antigen, measurement combined with some radioactive count again. The content of follicle stimulating hormone in the sample can be found from the standard curve.
(1) follicle stimulating hormone antiserum: 1 bottle of frozen dry product, add 10ml water to gently mix and recover, and put it to use after 10min. Buy 2 ~ 8 ℃ refrigerator can save 30 days.
(2) the I - 125 follicle-stimulating hormone: frozen one with 1 bottle, add 10 ml water recovery, gently inverted co-mingled 10 min after use, buy a refrigerator (2 ~ 8 ℃) can save 30 days (or reagent bottle date).
(3) follicle stimulating hormone standard: 7 bottles (a-g) of frozen dry goods were restored with water before use. In addition to the addition of 6ml to bottle A, the rest were added with 3ml each and placed for 30min before use. Recovery after follicle-stimulating hormone levels respectively 0, 2, 5, 10, 20, 40, 100 iu/L (2 nd WHO IRP - HMG) in the refrigerator (2 ~ 8 ℃) can be stored for 30 days.
(4) separation agent: 1 bottle, containing sheep antirabbit antipyknoglobulin and polyethylene glycol (PEG), in solution state, can be directly used for experiments. Opened the rear of 2 ~ 8 ℃ refrigerator can save 30 days. The separation agent must be thoroughly mixed before use.
Male: 1 ~ 7U/L.
Female: follicular stage: 1 ~ 9U/L.
Ovulation: 6 ~ 260U/L.
Luteal stage: 1 ~ 9U/L.
Menopause: 30 ~ 118U/L.
3.7 Clinical significance of laboratory results
Decrease: seen in estrogen and progesterone therapy, secondary sexual dysfunction, hypophysis, Sheehan syndrome, polycystic ovary syndrome, advanced pituitary dysfunction, etc.
Increase in testicular seminoma, crane felt (Klinefelter syndrome (Turner), Turner syndrome, primary amenorrhea, congenital aplasia ovary, use of adrenal cortical hormone treatment, primary reproductive hypofunction, ovarian sex fat, early adenohypophysis function already, giant cell degenerative lung cancer, etc.
(1) taking contraceptives and estrogen, pregnancy can affect the determination of serum follicle stimulating hormone.
(2)due to the large physiological changes of follicle stimulating hormone in serum, repeated determination should be made when judging and explaining a pathological phenomenon, so as to avoid misjudgment.
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